Infectious disorders can transmit from one person to another either directly or indirectly because they are brought on by pathogenic microorganisms such as bacteria, viruses, parasites, or fungus. Unfortunately, even in clinical laboratories, the diagnosis of infectious disorders is directly related to the length of time it takes to identify the pathogen using traditional culture methods, which can take anywhere from hours to days. Benefits of real-time PCR have evolved over the past ten years to become the industry standard for diagnosing infectious illnesses. Infectious disease specialists have championed real-time PCR because of its exceptional sensitivity, specificity, reproducibility, wide dynamic range, and speed of amplification for identifying organisms that cannot be grown in vitro or in situations where existing culture techniques are insensitive and/or require prolonged culture times.
