MonkeyPox Real Time PCR Detection Kit

Monkeypox virus (MPXV) is a zoonotic orthopoxvirus (OPX) endemic to west and central Africa. Each year, majority of human monkeypox (MPX) infections are reported from the Congo Basin in the Democratic Republic of Congo (DRC). MPXV is transmitted to humans from a primary zoonotic source, and the virus is also capable of human-to-human transmission. The animal reservoir of MPXV remains unknown; however, several genera of African rodents (Cricetomys, Graphiurus, Funiscirurus) have been identified as potential reservoirs responsible for transmission and maintenance of the virus. Contact with wildlife, which often occurs in rural communities in DRC that are dependent on bushmeat as a protein source, is thought to increase risk for human infection with MPXV.

The FastPlex MonkeyPox Real Time PCR detection kit is a real-time polymerase chain reaction (RT-PCR) test intended for the qualitative detection of MonkeyPox virus (MPXV) DNA in serum or lesion exudate samples. This kit is a real- time polymerase chain reaction (RT-PCR) test combined with Taqman fluorescent probe technology to qualitative detect the MonkeyPox virus DNA. The MPXV F3L gene DNA detection probes is fluorescently labeled with FAM, the MPXV B6R gene DNA detection probes is fluorescently labeled with HEX, and the internal control detection probe is fluorescently labeled with ROX or Cy5.

Results are for the detection of MPXV DNA. Positive results are indicative of the presence of MPXV DNA; clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of disease.

Negative results do not preclude MPXV virus infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information.

The FastPlex MonkeyPox Real Time PCR Detection kit is intended for use by trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures.

Features of RainSure Scientific Monkeypox Detection Kit:

Fast detection: In the fast amplification mode of the newly launched Fast-16 portable real-time PCR platform, the kit can obtain the detection results within 1 hour; with high specificity and sensitivity, it can detect low concentrations in samples Virus;

Wide coverage: The detection range of the kit can cover the West African branch and the Central African (Congo Basin) branch;

Convenient transportation: The kit provides two forms of reagent (lyophilized powder and liquid reagent). The lyophilized powder can be stored at room temperature for a long time, which is conducive to long-distance transportation and effectively reduces the transportation cost of reagents;

Fully compatible: real-time PCR equipment,

Large volume loading: up to 23 μL;

Three-gene joint inspection: F3L gene (FAM), B6R gene (HEX), internal reference gene (Cy5);

Sample detection limit: 500copies/ml (real-time fluorescent PCR)