PCR Assays

The PCR assays used in this study were based on the primers and conditions described in (Chen et al., 2011). The PCR assays were performed using a 25 μL reaction mix containing 2.5 μL of 10× PCR buffer, 0.2 μL of 10 mM dNTPs, 0.2 μL of each primer (10 μM), 0.2 μL of Taq polymerase (5 U/μL), and 20 μL of template DNA. The PCR amplification was performed using a thermocycler with the following program: 94°C for 3 min, followed by 35 cycles of 94°C for 30 sec, annealing at 55°C for 30 sec, and 72°C for 1 min, with a final extension step at 72°C for 10 min.