Real-time PCR (polymerase chain reaction) is a laboratory technique used to amplify and detect specific DNA sequences in a sample. It is a powerful and accurate tool used in molecular biology and genetic engineering to analyze gene expression, detect genetic mutations, and diagnose genetic diseases. The real-time PCR system consists of a thermal cycler, a software program, and a fluorescent detection system. The thermal cycler is used to amplify the target DNA sequence by heating and cooling the sample.
