Digital PCR principle is to partition single nucleic acid template into separate reaction chambers and performs a single molecule quantification by PCR amplification and fluorescence signal detection. The digital PCR technology does not reply on neither Ct values nor standard calibration curves, and has extremely high sensitivity, precision, and excellent repeatability. It is an absolute quantitative analysis technology that transcends real-time PCR.
The DropX-2000 Droplet Digital PCR System puts this powerful technology in your hands, ready to unveil new worlds of research at previously unattainable levels. The principal of digital PCR is partition and poisson distribution. The DNA or RNA amplicons are partitioned into more than 20,000 droplets. Each droplet contains 0 or 1 copy of amplicon. Upon completion of PCR thermal cycling, the droplets contain amplicon will show fluorescence, the droplets contain 0 copies will show no fluorescence. The number of fluorescent droplets will be counted as “0” or “1”. The copy number (concentration) of the sample can be calculated by the number of fluorescent droplets through poisson distribution. distribution. The DropX-2000 System uses advanced microfluidics technology to achieve sample partition, generating about 20,000 nanoliter-sized droplets per sample.
The DropX-2000 system is suitable for tumor biomarker research, copy number variation analysis, viral pathogen detection, microbial detection, genetically modified organism detection, mRNA and miRNA detection, rare nucleic acid sample detection, NGS reference standard quality control, gene editing result verification, etc.
Cartidge Scanner Product Description
Using a superb optical design, the fluorescent signal of a droplet can be read accurately and quickly by the scanner. The number of positive or negative droplets is identified by the imaging software. Absolute quantification of the sample can be achieved by statistical calculation of Poisson distribution. The cartridge scanner is available in 8/16/32 samples throughput models.
Industry-leading multi-fluorescence channel design enhances the function of digital PCR system for multiplex target detection, multi-channel fluorescence signal acquisition. Fast and integrated automated image analysis system.
Instrument Model List
SG8-3000 （8 Throughput）
SG-2000 （16 Throughput ）
SG32-2000 （32 Throughput ）
Dscanner3-1000(Three color fluorescence)
Dscanner4-1000(Four color fluorescence)
Dscanner5-1000(Five color fluorescence)
Droplets per reaction
PCR Thermal Cycling
40PCR ＜ 90 minutes （max heating 10℃/S ,max cooling 6℃/S ）
3 channel FAM/SYBR Green, HEX/VIC, CY5
4 channel FAM/SYBR Green, HEX/VIC, ROX and CY5
5 channel FAM/SYBR Green, HEX/VIC, ROX ，CY5 and CY 5.5
Add On Features
Can be upgraded to an integrated system with a robotic arm to eliminate the manual transfer of microfluidic chips from the sample preparation station to the chip scanner.
Sample Throughput Per Run