The principle of digital PCR is to partition a single nucleic acid template into separate reaction chambers and perform single molecule quantification through PCR amplification and fluorescence signal detection. Digital PCR technology does not rely on Ct values or standard calibration curves. Instead, it offers extremely high sensitivity, precision, and excellent repeatability. It is an absolute quantitative analysis technology that surpasses real-time PCR.
The DropX-2000 Droplet Digital PCR System puts this powerful technology in your hands, enabling the exploration of new realms of research at previously unattainable levels. The principle of digital PCR involves partitioning and Poisson distribution. DNA or RNA amplicons are divided into more than 20,000 droplets, with each droplet containing either 0 or 1 copy of the amplicon. Following PCR thermal cycling, droplets containing amplicons exhibit fluorescence, while those without copies show no fluorescence. The number of fluorescent droplets is then counted as either "0" or "1." The copy number (concentration) of the sample can be calculated using the number of fluorescent droplets through Poisson distribution. The DropX-2000 System utilizes advanced microfluidics technology to achieve sample partitioning, generating approximately 20,000 nanoliter-sized droplets per sample.
The DropX-2000 system is suitable for various applications, including tumor biomarker research, copy number variation analysis, viral pathogen detection, microbial detection, genetically modified organism detection, mRNA and miRNA detection, rare nucleic acid sample detection, NGS reference standard quality control, gene editing result verification, and more.
The PCR Amplifying Apparatus SG-2000, which utilizes patented microfluidic technology and a unique reagent system, enables rapid achievement of Poisson distribution of samples in the droplet preparation process. It is fully automated with one-button operation. The station incorporates patented temperature control components for top performance, along with the RainSure temperature control management system. With the integrated digital PCR cartridge, it achieves swift amplification of nucleic acid samples.
It combines a high-precision, pressure-controlled microfluidic system with a microfluidic cartridge to rapidly and consistently generate uniform microemulsion droplets. With stable and long-lasting performance, it can produce 20,000 droplets in 70 seconds, ensuring:
Uniform droplet size with a coefficient of variation (CV) of ≤ 3%.
Droplet diameter adjustable within the range of 70-110 microns based on user requirements.
The system adopts an innovative flat surface heating design, enabling rapid temperature increase and cooling. The fastest heat ramp rate reaches 10°C/s, while the fastest cooling ramp rate reaches 6°C/s. This allows for the completion of 40 PCR thermal cycles within 90 minutes.
（16 Samples ）
（32 Samples ）
Dscanner3-1000 (Three-color fluorescence)
Dscanner4-1000 (Four-color fluorescence)
Dscanner5-1000 (Five-color fluorescence)
Droplets per Reaction
PCR Thermal Cycling
40 cycles in＜ 90 minutes （Max heating 10℃/s , max cooling 6℃/s ）
It can be upgraded to an integrated system with a robotic arm, eliminating the need for manual transfer of cartridges from the sample preparation station to the cartrudge scanner.
Sample Throughput per Run
8/ 16/ 32