Human KRAS Gene Mutation Detection Kit

This kit is used to detect KRAS (V-Ki-ras2 Kirsten ratsarcoma viral oncogene homolog) G12D mutation in tissue DNA and cfDNA of plasma samples. The K-ras gene (K-ras, p21) family has three genes associated with human tumors--H-ras, K-ras and N-ras, which are located on chromosomes 11, 12 and 1, respectively. 

The K-ras gene is also known as the p21 gene because it encodes a 21kD ras protein. Of the ras genes, K-Ras, which has the greatest impact on human cancers, acts like a molecular switch: when normal, it controls the pathway that regulates cell growth; when abnormal, it causes the cell to continue growing and prevents the cell from self-destruction. She is involved in intracellular signal transmission. When the K-ras gene is mutated, the gene is permanently activated, and normal ras protein cannot be produced, resulting in disordered intracellular signaling, uncontrolled cell proliferation and cancer. 

About 30% of human malignant tumors are related to RAS gene mutation, and the product of ras mutation can always be in an active state. Mutations in k-ras are common in leukemia, lung, rectal, and pancreatic cancer, with mutations in 30%-35% of rectal cancers. 96% of KRAS gene mutations occur at codons 12 and 13 of exon 2, and KRAS G12D mutations account for about 17-18%.

(includes primer probes only)