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A Aerosol refers to the vaporized PCR reaction solution, which diffuses into the air and causes potential environmental pollution. RainSure adopts the water-in-oil technology, the PCR reaction is wrapped for reaction, and the closed state is formed by the sealing cover , which effectively avoids aerosol pollution .
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A It is suitable for peripheral blood samples collected by EDTA anticoagulation or citrate anticoagulation. The number of white blood cells in the sample is not less than 10 7 . For patients with normal white blood cell count, no less than 5ml of peripheral blood is drawn. Samples should be transported and stored at 2-8 °C after collection, and processed within 24 hours; if long-term storage is required, they should be stored at -70 °C and below, and the storage period should not exceed 1 month.
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A RainSure digital PCR system can be used for detection, but it needs to be divided into two wells for detection, so it is not recommended to use single-color fluorescence, because the manual operation of the process is easy to cause errors.
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A It is not recommended to use qPCR reagents and temperature reaction systems to directly perform digital PCR experiments , because the enzyme amplified by digital PCR requires an initial denaturation temperature of 98°C for 10 minutes, and the requirements for the concentration of primers and probes are also different. Conditions were optimized for different probes and concentrations.
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A Considering the two aspects of reagent efficiency and ease of operation, it is recommended to add the system after mixing.
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A No, the reverse transcription method (probe method) premix can be used in conventional probe method experiments , but the cost is high, and vice versa.
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A Taq enzyme, magnesium ion, dNTP, mainly because the application ratio of each component is different.
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A It can be used for the detection and application of animals, plants and microorganisms at the same time.
