The principle of Digital PCR is to divide nucleic acids template into separate reaction chambers, allowing for individual molecule quantification through PCR amplification and fluorescence signal detection. Unlike real-time PCR, digital PCR does not rely on Ct values or standard calibration curves. It offers exceptionally high sensitivity, precision, and repeatability, making it an absolute quantitative analysis technology.
The DropX-2000 Droplet Digital PCR System puts this powerful technology at your disposal, enabling groundbreaking research at previously unattainable levels. The core principle of digital PCR is partitioning and Poisson distribution. DNA or RNA amplicons are divided into more than 20,000 droplets, with each droplet containing either 0 or 1 copy of the amplicon. After the completion of PCR thermal cycling, the droplets containing amplicons exhibit fluorescence, while those without copies do not. The number of fluorescent droplets is then counted as "0" or "1". By employing Poisson distribution, the concentration (copy number) of the sample can be calculated based on the number of fluorescent droplets. The DropX-2000 System utilizes advanced microfluidics technology to achieve sample partitioning, generating approximately 20,000 nanoliter-sized droplets per sample.
The DropX-2000 system is well-suited for various applications, including tumor biomarker research, copy number variation analysis, viral pathogen detection, microbial detection, genetically modified organism detection, mRNA and miRNA detection, rare nucleic acid sample detection, NGS reference standard quality control, and gene editing result verification.
Equipped with an exceptional optical design, the cartridge scanner accurately and rapidly reads the fluorescent signal of each droplet. The imaging software identifies the number of positive or negative droplets, enabling absolute quantification of the sample through statistical calculations based on the Poisson distribution. The biochip scanner is available in 3/4/5-color models.
The industry-leading multi-fluorescence channel design enhances the functionality of the digital PCR system, facilitating multiplex target detection and acquisition of multi-channel fluorescence signals. Additionally, the system incorporates a fast and integrated automated image analysis system.
（16 Samples ）
（32 Samples ）
Dscanner3-1000 (Three-color fluorescence)
Dscanner4-1000 (Four-color fluorescence)
Dscanner5-1000 (Five-color fluorescence)
3 channels: FAM/SYBR Green, HEX/VIC, and CY5
4 channels: FAM/SYBR Green, HEX/VIC, ROX, and CY5
5 channels: FAM/SYBR Green, HEX/VIC, ROX, CY5, and CY5.5
It can be upgraded to an integrated system with a robotic arm, eliminating the need for manual transfer of cartridges from the sample preparation station to the cartrudge scanner.
Sample Throughput per Run